CST, 39296T, NLRP12 (E8A3C) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying NLRP12. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, NLRP12 (E8A3C) Rabbit Monoclonal Antibody (CST #39296) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity NLRP12 (E8A3C) Rabbit Monoclonal Antibody recognizes endogenous levels of total NLRP12 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln190 of human NLRP12 protein. Background NLR family pyrin domain containing 12 (NLRP12, NALP12, PYPAF7, MONARCH-1), a member of the NOD-like receptor (NLR) family, is an intracellular protein that plays a role in forming an inflammasome and can also act as a regulator of inflammatory signaling (1). NLRP12 is expressed in dendritic cells, neutrophils, granulocytes, and macrophages (2). Like other NLR family members, NLRP12 is composed of a pyrin domain (PYD), a nucleotide-binding domain (NBD), and a leucine-rich repeat (LRR) domain (2). NLRP12 functions as a negative regulator of canonical NFκB signaling by inhibiting accumulation of hyperphosphorylated IRAK1 downstream of TLR signaling (3). NLRP12 also acts as a negative regulator of non-canonical NFκB signaling by associating with NFκB-inducing kinase (NIK) and TRAF3, which leads to proteasomal degradation of NIK (4, 5). During Yersinia pestis infection, NLRP12 acts as an inflammasome component, resulting in the release of IL-1β and IL-18 (6). NLRP12 plays a role in regulating gut microbiota, reducing susceptibility to colitis (7). Alternate Names CLR19.3; FCAS2; monarch 1; Monarch-1; NACHT, leucine rich repeat and PYD containing 12; NACHT, LRR and PYD containing protein 12; NACHT, LRR and PYD domains-containing protein 12; NAL12; NALP12; NLR family pyrin domain containing 12; NLR family, pyrin domain containing 12; NLRP12; nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 12; PAN6; PYPAF7; PYRIN-containing APAF1-like protein 7; Regulated by nitric oxide; RNO; RNO2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 100-110 Source/Isotype: Rabbit IgG