CST, 39300T, CD5 (E8X3S) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CD5. Validated for WB,IP,IHC,IHC. Available in 2 sizes. Highly specific and rigorously validated in-house, CD5 (E8X3S) Rabbit Monoclonal Antibody (CST #39300) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 IHC Leica Bond: 1:400 Immunohistochemistry (Paraffin): 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier-free (BSA and azide free) version of this product see product # 86389 . Protocol Available protocols: Western Blotting, Immunoprecipitation, IHC Leica Bond, Immunohistochemistry (Paraffin) Specificity / Sensitivity CD5 (E8X3S) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD5 protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly219 of human CD5 protein. Background CD5 is a type-I transmembrane protein belonging to the scavenger receptor cysteine-rich (SRCR) family, characterized by the presence of at least one SRCR domain of 90-110 amino acids. CD5 is expressed by all mature T cells, the B-1a subset of mature B cells, and some leukemic B cells. Its expression is increased in regulatory T and B cells (Tregs/Bregs). Anergic T and B cells also have elevated CD5 expression. Elevated levels of CD5 are also found in many autoimmune disorders (1-3). CD5 is associated with the T cell receptor (TCR) and negatively modulates T cell activation and differentiation. CD5 expression on the tumor infiltrating T lymphocytes is inversely correlated with their antitumor activity (4-6). Recently, it was reported that CD5 directly binds to IL6 and can mediate downstream signaling. CD5+ B cells promote tumor growth in animal models (7). Reagents targeting CD5 have been actively pursued as therapeutic interventions for cancer and other conditions (8,9). Alternate Names CD5; CD5 antigen (p56-62); CD5 molecule; epididymis secretory sperm binding protein; LEU1; Lymphocyte antigen T1/Leu-1; T-cell surface glycoprotein CD5; T1 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 65 Source/Isotype: Rabbit IgG