CST, 39370T, Rad51D (F6U3A) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying RAD51L3. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Rad51D (F6U3A) Rabbit Monoclonal Antibody (CST #39370) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Rad51D (F6U3A) Rabbit Monoclonal Antibody recognizes endogenous levels of total Rad51D protein. This antibody detects an approximately 80 kDa protein of unknown identity in some cell lines. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human Rad51D protein. Background Rad51 paralog D (Rad51D) is one of five Rad51 paralogs that form two distinct complexes, BCDX2 (Rad51B, Rad51C, Rad51D, and XRCC2) and CX3 (Rad51C and XRCC3) (1). Along with Rad51, these complexes are crucial in the repair of DNA double-strand breaks through homologous recombination (2), and in the response to DNA replication stress (3,4). Germline mutations in homologous recombination-associated genes, including those encoding Rad51C and Rad51D, lead to increased susceptibility to breast, ovarian, prostate, and pancreatic cancer (5). Alternate Names BROVCA4; DNA repair protein RAD51 homolog 4; HsTRAD; R51H3; RA51D; RAD51 homolog D; RAD51 paralog D; RAD51-like 3 (S. cerevisiae); RAD51-like protein 3; RAD51D; RAD51L3; recombination repair protein; TRAD Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 30 Source/Isotype: Rabbit IgG