CST, 39413SF, VWF (D8L8G) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying VWF. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, VWF (D8L8G) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #39413) is ready to ship. Product Usage Information This product is the carrier free version of product #65707. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 65707 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity VWF (D8L8G) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total VWF protein. This antibody recognizes both the VWF precursor and mature VWF. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant human VWF protein. Background VWF (Von Willebrand factor) is a multimeric plasma glycoprotein that promotes adhesion of platelets to sites of vascular injury (1). Mature circulating VWF is made up of disulfide-bonded multimers that are in a complex with factor VIII (2). VWF is stored in secretory Weibel-Palade bodies in endothelial cells (3,4). It is synthesized as a large precursor protein and undergoes extensive posttranslational modifications including dimerization in the endoplasmic reticulum followed by cleavage of the pro-peptide and multimerization in the Golgi apparatus (3,4). VWF is important in hemostasis, and genetic defects in the structure and modification of VWF can cause von Willebrand disease (VWD), the most common congenital bleeding disorder in humans (5). Alternatively, increased levels of VWF have been shown to be involved in acute coronary thrombosis and are a clinical risk marker for atherosclerosis (6). VWF has also been shown to have a role in inflammation, functioning as an adhesive site for a variety of leukocyte subsets (7). Through siRNA experiments and the use VWF-deficient mice, it has also been shown that VWF regulates angiogenesis (8). Alternate Names coagulation factor VIII VWF; F8VWF; von Willebrand antigen 2; von Willebrand antigen II; von Willebrand factor; VWD; VWF Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 310, 460 Source/Isotype: Rabbit IgG