CST, 39443S, CD69 (H1.2F3) Hamster Monoclonal Antibody (FITC Conjugate)

Monoclonal Antibody for studying CD69 mouse. Validated for Immunofluorescence (Frozen),Flow Cytometry (Fixed/Permeabilized),Flow Cytometry (Live). Highly specific and rigorously validated in-house, CD69 (H1.2F3) Hamster Monoclonal Antibody (FITC Conjugate) (CST #39443) is ready to ship. Product Usage Information For optimal flow cytometry results, we recommend 0.25 μg of antibody per test. Immunofluorescence (Frozen): 1:400 - 1:800 Flow Cytometry (Fixed/Permeabilized): 1:200 Flow Cytometry (Live): 1:200 Storage Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. This product is stable for 12 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Immunofluorescence (Frozen), Flow Cytometry (Fixed/Permeabilized), Flow Cytometry (Live) Specificity / Sensitivity CD69 (H1.2F3) Hamster Monoclonal Antibody (FITC Conjugate) recognizes endogenous levels of total CD69 protein. This antibody conjugate detects an epitope within the extracellular domain. Species Reactivity: Mouse Source / Purification This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. Background CD69, also known as Leu-23, is a type II transmembrane glycoprotein that is expressed on the surface of T cells, B cells, and NK cells (1,2). This phosphorylated disulfide-linked 28 to 32-kDa homodimer is constitutively expressed on a subset of thymocytes and platelets. It also acts as an activation antigen of lymphocytes, NK cells, neutrophils, and eosinophils (1-6). Studies have shown that stimulation of the T cell receptor (TCR) increases the expression of CD69 on the cell surface. The ability to detect the level of CD69 expression after TCR activation makes CD69 an ideal indicator of T cell activation (1). The H1.2F3 antibody is widely used as a marker for T cell activation (7). Alternate Names 5830438K24Rik; AI452015; AIM; Cd69; CD69 antigen; Early activation antigen CD69; VEA; Very Early Activation Antigen Specification REACTIVITY: M SENSITIVITY: Endogenous Source/Isotype: Hamster (armenian) IgG