CST, 39475T, IL-2R alpha/CD25 (E9W2J) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying IL2RA mouse. Validated for WB,IP,IHC,IHC,IF,F,F. Available in 2 sizes. Highly specific and rigorously validated in-house, IL-2R alpha/CD25 (E9W2J) Rabbit Monoclonal Antibody (CST #39475) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 IHC Leica Bond: 1:50 - 1:200 Immunohistochemistry (Paraffin): 1:50 - 1:200 Immunofluorescence (Frozen): 1:800 Flow Cytometry (Fixed/Permeabilized): 1:100 - 1:400 Flow Cytometry (Live): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier-free (BSA and azide free) version of this product see product # 95742 . Protocol Available protocols: Western Blotting, Immunoprecipitation, IHC Leica Bond, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Flow Cytometry (Fixed/Permeabilized), Flow Cytometry (Live) Specificity / Sensitivity IL-2R alpha/CD25 (E9W2J) Rabbit Monoclonal Antibody recognizes endogenous levels of total IL-2Rα/CD25 protein. This antibody non-specifically labels myenteric nerves in the gut. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of mouse IL-2Rα/CD25 protein. Background Interleukin-2 (IL-2) is a T cell stimulatory cytokine best known for inducing T cell proliferation and NK cell proliferation and activation (1,2). IL-2 also promotes peripheral development of regulatory T cells (Tregs) (3,4). Conversely, IL-2 is involved in the activation-induced cell death (AICD) that is observed post T cell expansion by increasing levels of Fas on CD4 T cells (5). The effects of IL-2 are mediated through a trimeric receptor complex consisting of IL-2Rα, IL-2Rβ, and the common gamma chain, γc (1,2). IL-2Rα binds exclusively to IL-2 with low affinity and increases the binding affinity of the whole receptor complex including IL-2Rβ and γc subunits. IL-15 also binds to IL-2Rβ (1,2). γc is used by other cytokines, including IL-4, IL-7, IL-9, IL-15, and IL-21 (1,2). Binding of IL-2 initiates signaling cascades involving Jak1, Jak3, Stat5, and the PI3K/Akt pathways (1,2). Alternate Names CD25; IL-2 receptor subunit alpha; IL-2-RA; IL-2R alpha chain; IL-2R subunit alpha; IL2-RA; Il2r; Il2ra; interleukin 2 receptor, alpha chain; Interleukin-2 receptor subunit alpha; Ly-4; Ly-43; MGC130561; OTTMUSP00000012002; p55; p55 chain Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 40-80 Source/Isotype: Rabbit IgG