CST, 39517S, ATP6V1A (E5N9E) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ATP6V1A. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, ATP6V1A (E5N9E) Rabbit Monoclonal Antibody (CST #39517) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity ATP6V1A (E5N9E) Rabbit Monoclonal Antibody recognizes endogenous levels of total ATP6V1A protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human ATP6V1A protein. Background Eukaryotic cells contain ATP-driven proton pumps known as vacuolar H+-ATPases (V-ATPases) that acidify intracellular compartments and translocate protons across the plasma membrane (1,2). Intracellular V-ATPases play an important role in endocytosis and intracellular membrane trafficking, while plasma membrane V-ATPases are important in processes such as urinary acidification and bone resorption (1,2). Vacuolar ATPase enzymes are large, heteromultimeric protein complexes with component proteins found in either the V1 peripheral domain or the V0 integral domain (2). The cytoplasmic V1 domain contains a hexamer of A and B catalytic subunits, as well as a number of other protein subunits required for ATPase assembly and ATP hydrolysis. The integral V0 V-ATPase domain exhibits protein translocase activity and is responsible for transport of protons across the membrane (2). Research studies show that the V-ATPase subunits ATP6V0c, ATP6V0d1, ATP6V1A, ATP6V1B2, and ATP6V1D interact with the Ragulator protein complex and are essential for amino acid induced activation of mTORC1 on the surface of lysosomes (3). Additionally, ATP6V1A has been shown to interact with SARS-CoV-2 M protein and facilitate viral infection (4,5). Alternate Names ARCL2D; ATP6A1; ATP6V1A; ATP6V1A1; ATPase H+ transporting V1 subunit A; ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A; ATPase, H+ transporting, lysosomal, subunit A1; H(+)-transporting two-sector ATPase, subunit A; H+-transporting ATPase chain A, vacuolar (VA68 type); HO68; IECEE3; V-ATPase 69 kDa subunit; V-ATPase 69 kDa subunit 1; V-ATPase A subunit 1; V-ATPase subunit A; V-type proton ATPase catalytic subunit A; VA68; vacuolar ATP synthase catalytic subunit A, ubiquitous isoform; Vacuolar ATPase isoform VA68; vacuolar proton pump alpha subunit 1; Vacuolar proton pump subunit alpha; VATA; Vma1; VPP2 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 75 Source/Isotype: Rabbit IgG