CST, 39539SF, Phospho-CAD (Ser1859) (D5K5W) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying CAD (Ser1859) phosphate. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, Phospho-CAD (Ser1859) (D5K5W) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #39539) is ready to ship. Product Usage Information This product is the carrier free version of product #67235. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 67235 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Phospho-CAD (Ser1859) (D5K5W) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of CAD protein only when phosphorylated at Ser1859. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser1859 of human CAD protein. Background CAD is essential for the synthesis of pyrimidine nucleotides and possesses the following enzymatic activities: glutamine amidotransferase, carbamoyl-phosphate synthetase, aspartate transcarbamoylase, and dihydroorotase. Thus, the enzyme converts glutamine to uridine monophosphate, a common precursor of all pyrimidine bases, and it is necessary for nucleic acid synthesis (1). In resting cells, CAD is localized mainly in the cytoplasm where it carries out pyrimidine synthesis. As proliferating cells enter S phase, MAP Kinase (Erk1/2) phosphorlyates CAD at Thr456, resulting in CAD translocation to the nucleus. As cells exit S phase, CAD is dephosphorylated at Thr456 and phosphorylated at Ser1406 by PKA, returning the pathway to basal activity (2). Various research studies have shown increased expression of CAD in several types of cancer, prompting the development of pharmacological inhibitors such as PALA. Further studies have identified CAD as a potential predictive early marker of prostate cancer relapse (3). mTORC1 is a protein kinase that works to regulate the growth and proliferation of cells by sensing and integrating various growth signals. S6 kinase 1 (S6K1) is a downstream ribosomal protein target of mTORC1 and directly phosphorylates Ser1859 on CAD. This phosphorylation stimulates the first three steps of the pyrimidine synthesis and thus helps to advance the cells overall progression through S phase of the cell cycle (4,5). Alternate Names Aspartate carbamoyltransferase; CAD; CAD protein; CAD trifunctional protein; carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase; CDG1Z; Dihydroorotase; EIEE50; GATD4; Glutamine-dependent carbamoyl-phosphate synthase; multifunctional protein CAD; PYR1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 240 Source/Isotype: Rabbit IgG