CST, 3958P, Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit Monoclonal Antibody (Biotinylated)

Monoclonal Antibody for studying MEK2 (Ser222/Ser226) phosphate/MEK1 (Ser218/Ser222) phosphate. Validated for Western Blotting,Peptide ELISA (DELFIA). Highly specific and rigorously validated in-house, Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit Monoclonal Antibody (Biotinylated) (CST #3958) is ready to ship. Product Usage Information Biotinylated antibodies are ideal for immunoassay technologies and high-throughput ELISA platforms that require antibody pairs where both antibodies are from the same host. Platforms utilizing biotinylated antibodies include, but are not limited to, MSD, xMAP, Quanterix Simoa, AlphaLISA, AlphaScreen, HTRF, LANCE, and TR-FRET. Optimal dilutions/working concentrations should be determined by the end user. Please contact us if you require the antibody clone biotinylated at a different concentration, a carrier-free formulation, or a more customized packaging solution. Storage Supplied in 140 mM NaCl, 3 mM KCl, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit Monoclonal Antibody (Biotinylated) detects endogenous levels of MEK1/2 only when phosphorylated at Ser217/221. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser217/221 of human MEK1/2. Background MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC-12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII. Alternate Names CFC3; CFC4; Dual specificity mitogen-activated protein kinase kinase 1; Dual specificity mitogen-activated protein kinase kinase 2; ERK activator kinase 1; ERK activator kinase 2; FLJ26075; MAP kinase kinase 1; MAP kinase kinase 2; MAP2K1; MAP2K2; MAPK/ERK kinase 1; MAPK/ERK kinase 2; MAPKK 1; MAPKK 2; MAPKK1; MAPKK2; MEK 1; MEK 2; MEK1; MEK2; MEL; mitogen-activated protein kinase kinase 1; mitogen-activated protein kinase kinase 2; mitogen-activated protein kinase kinase 2, p45; MKK1; MKK2; MP2K1; MP2K2; PRKMK1; PRKMK2; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1) Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 45 Source/Isotype: Rabbit IgG