CST, 3960T, Nur77 (D63C5) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Nur77. Validated for WB,IP,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, Nur77 (D63C5) Rabbit Monoclonal Antibody (CST #3960) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunofluorescence (Immunocytochemistry): 1:100 - 1:200 Flow Cytometry (Fixed/Permeabilized): 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Nur77 (D63C5) Rabbit Monoclonal Antibody detects endogenous levels of total human Nur77 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu535 of human Nur77. Background Nur77, also known as TR3 and NGFI-B, is an immediate-early response gene and an orphan member of the steroid/thyroid/retinoid receptor superfamily (1-3). Nur77 is composed of an amino-terminal transactivation domain, a central DNA-binding domain and a carboxy-terminal ligand-binding domain. Expression of Nur77 is rapidly induced by a variety of stimuli, including apoptotic, mitogenic and stress signals (1-6). It has been proposed to have many functions related to cell proliferation, differentiation and apoptosis. Nur77 has been extensively studied in T cells where it has been implicated in the process of negative selection and TCR-mediated apoptosis (5,6). Nur77 binds to specific DNA elements leading to the regulation of target genes (7). As a possible mechanism for regulating apoptosis, Nur77 can induce the expression of apoptotic genes such as FasL and TRAIL (8,9). Nur77 is heavily phosphorylated by multiple kinases, which may affect its transactivation activity as well as its subcellular localization (4,10,11). Translocation of Nur77 from the nucleus to the mitochondria can regulate its association with Bcl-2 and control the release of cytochrome c, thereby triggering apoptosis (12,13). Alternate Names Early response protein NAK1; GFRP1; growth factor-inducible nuclear protein N10; HMR; hormone receptor; MGC9485; N10; NAK-1; NAK1; nerve growth factor IB nuclear receptor variant 1; NGFIB; NP10; NR4A1; Nuclear hormone receptor NUR/77; Nuclear receptor subfamily 4 group A member 1; nuclear receptor subfamily 4, group A, member 1; Nuclear receptor subfamily 4immunitygroup A member 1; Nur77; Orphan nuclear receptor HMR; Orphan nuclear receptor TR3; ST-59; steroid receptor TR3; Testicular receptor 3; TR3; TR3 orphan receptor Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 70-80 Source/Isotype: Rabbit IgG