CST, 39636S, TPPP/p25 Antibody

Polyclonal Antibody for studying TPPP mouse. Validated for Western Blotting. Highly specific and rigorously validated in-house, TPPP/p25 Antibody (CST #39636) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody . Protocol Available protocols: Western Blotting Specificity / Sensitivity TPPP/p25 Antibody recognizes endogenous levels of total TPPP/p25 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu194 of mouse TPPP/p25 protein. Antibodies are purified by peptide affinity chromatography. Background Tubulin polymerization-promoting protein (TPPP, also known as p25) is a microtubule nucleation factor in oligodendrocytes. As a largely unstructured protein, TPPP/p25 induces microtubule polymerization (1,2). TPPP/p25 is enriched in oligodendrocytes in the brain and may play a unique role in the structure and function of these unique cells (3,4). Oligodendrocytes extend elaborate microtubule arbors that contact neuronal axon segments to generate a myelin sheath that is critical for proper propagation of axonal action potentials. TPPP/p25 may not only play a critical role in establishing the microtubule network for oligodendrocytes, but may also contribute to the generation of Golgi outposts, which are Golgi found outside of the cell body. Golgi outposts may play a specialized role in building the local cytoarchitecture of oligodendrocytes and other brain cells (5,6). Misregulation of TPPP/p25 is linked to several neurodegenerative diseases, including Parkinson's disease, and may contribute to disease etiology and/or represent a potential therapeutic target (7). Alternate Names 25 kDa brain-specific protein; 2900041A09Rik; AI849835; brain-specific protein p25 alpha; p25-alpha; TPPP; TPPP/p25; tubulin polymerization promoting protein; Tubulin polymerization-promoting protein Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 25 SOURCE: Rabbit