CST, 3965S, Ras Antibody

Polyclonal Antibody for studying HRas/MRas/RRas/NRas/KRas. Validated for Western Blotting. Highly specific and rigorously validated in-house, Ras Antibody (CST #3965) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Ras Antibody detects endogenous levels of total K-Ras, H-Ras, and N-Ras. This antibody may also cross-react with R-Ras and M-Ras. Species Reactivity: Human, Mouse, Rat, Monkey, D. melanogaster, Pig, S. cerevisiae Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues close to the amino-terminus of human K-Ras. Antibodies are purified by protein A and peptide affinity chromatography. Background The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras, and N-Ras) cycle between active (GTP-bound) and inactive (GDP-bound) forms (1). Receptor tyrosine kinases and G protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAPK pathway (2-4). GTPase-activating proteins (GAPs) normally facilitate the inactivation of Ras. However, research studies have shown that in 30% of human tumors, point mutations in Ras prevent the GAP-mediated inhibition of this pathway (5). The most common oncogenic Ras mutation found in tumors is Gly12 to Asp12 (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein (5,6). Alternate Names 'C-K-RAS; ALPS4; C-BAS/HAS; c-H-ras; C-HA-RAS1; c-has/bas p21 protein; c-K-ras; c-Ki-ras; c-Ki-ras2; c-Kirsten-ras protein; c-ras-Ki-2 activated oncogene; cellular c-Ki-ras2 proto-oncogene; cellular transforming proto-oncogene; CFC2; CMNS; CTLO; FLJ42964; GTP- and GDP-binding peptide B; GTPase HRas; GTPase HRas, N-terminally processed; GTPase KRas; GTPase KRas, N-terminally processed; GTPase NRas; H-Ras; H-Ras-1; H-RASIDX; Ha-Ras; Ha-Ras1 proto-oncoprotein; HAMSV; Harvey rat sarcoma viral oncogene homolog; Harvey rat sarcoma viral oncoprotein; HRAS; HRas proto-oncogene, GTPase; HRAS1; K-Ras; K-RAS; K-Ras 2; K-ras p21 protein; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; Ki-Ras; Kirsten rat sarcoma viral oncogene homolog; Kirsten rat sarcoma viral proto-oncogene; Kirsten rat sarcoma-2 viral (v-Ki-ras2) oncogene homolog; KRAS; KRAS proto-oncogene, GTPase; KRAS1; KRAS2; M-RAs; MRAS; muscle and microspikes RAS; muscle RAS oncogene homolog; N-ras; N-RAS; N-ras protein part 4; NCMS; neuroblastoma RAS viral (v-ras) oncogene homolog; neuroblastoma RAS viral oncogene homolog; NRAS; NRAS proto-oncogene, GTPase; NRAS1; NS; NS11; NS3; NS6; OES; oncogene KRAS2; Oncogene RRAS; p19 H-RasIDX protein; p21ras; p23; PR310 c-K-ras oncogene; R-Ras; R-RAS3; RALD; Ras family small GTP binding protein H-Ras; ras family small GTP binding protein R-Ras; RAS related; Ras-related protein M-Ras; Ras-related protein R-Ras; Ras-related protein R-Ras3; RASH; RASH1; RASK; RASK2; RASM; RASN; related RAS viral (r-ras) oncogene homolog; RRAS; RRAS3; transformation gene: oncogene HAMSV; Transforming protein N-Ras; transforming protein p21; Transforming protein p21; v-Ha-ras Harvey rat sarcoma viral oncogene homolog; v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog; v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog; v-ras neuroblastoma RAS viral oncogene homolog Specification REACTIVITY: H M R Mk Dm Pg Sc SENSITIVITY: Endogenous MW (kDa): 21 SOURCE: Rabbit