CST, 3966S, TRRAP (D2966) Antibody

Polyclonal Antibody for studying TRRAP. Validated for Western Blotting. Highly specific and rigorously validated in-house, TRRAP (D2966) Antibody (CST #3966) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity TRRAP (D2966) Antibody detects endogenous levels of total TRRAP protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the human TRRAP protein. Antibodies are purified by peptide affinity chromatography. Background Transformation/transcription domain-associated protein (TRRAP) is a highly conserved 434 kDa protein found in various multiprotein complexes, such as SAGA, PCAF, NuA4 and TIP60, which contain histone acetyltransferase (HAT) activity (1-4). TRRAP functions as an adaptor protein by binding directly to the transactivation domains of transcriptional activator proteins and facilitating the recruitment of HAT complexes to acetylate histone proteins and activate transcription (1-5). TRRAP is required for the transcriptional activation and cell transformation activities of c-Myc, E2F1, E2F4, p53 and the adenovirus E1A proteins (1,6,7). TRRAP is also essential in early development and is required at the mitotic checkpoint and for normal cell cycle progression (8,9). In addition, TRRAP has been shown to function in DNA repair. As part of the TIP60 complex, TRRAP is required for the acetylation of histone H4 at double-stranded DNA breaks and subsequent DNA repair by homologous recombination (10). In addition, TRRAP associates with the MRN (MRE11, RAD50, NBS1) complex, which lacks intrinsic HAT activity yet functions in the sensing and subsequent repair of double-stranded breaks by non-homologous DNA end-joining (11). TRRAP shows significant homology to the PI-3 kinase domain of the ATM family of kinases; however, amino acids that map to the catalytic site of the kinase domain are not conserved in TRRAP (1). Alternate Names 350/400 kDa PCAF-associated factor; DEDDFA; DFNA75; FLJ10671; PAF350/400; PAF400; STAF40; TR-AP; Tra1; Tra1 homolog; transformation/transcription domain associated protein; Transformation/transcription domain-associated protein; TRRAP Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 434 SOURCE: Rabbit