CST, 3972S, GRB2 Antibody

Polyclonal Antibody for studying Grb2. Validated for Western Blotting. Highly specific and rigorously validated in-house, GRB2 Antibody (CST #3972) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity GRB2 Antibody detects endogenous levels of total GRB2 protein. Species Reactivity: Human, Mouse, Rat, Hamster, Monkey, Bovine Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to carboxy-terminal residues of human GRB2. Antibodies are purified by protein A and peptide affinity chromatography. Background Growth factor receptor-binding protein 2 (GRB2) is an adaptor protein that is involved in RTK signal transduction. The SH2 domain of GRB2 binds to tyrosine phosphorylated proteins, such as EGFR, IRS-1, Shc, and Gab1 (1). The SH3 domain of GRB2 associates with SOS, which stimulates the Ras GTP-binding activity, leading to the activation of the MAP kinase and other signaling pathways. Phosphorylation of GRB2 at Tyr209 by Bcr-Abl and EGFR abolishes its association with SOS and negatively regulates downstream signaling (2). Alternate Names abundant SRC homology; Adapter protein GRB2; ASH; EGFRBP-GRB2; epidermal growth factor receptor-binding protein GRB2; epididymis secretory sperm binding protein; GRB2; Grb3-3; growth factor receptor bound protein 2; Growth factor receptor-bound protein 2; growth factor receptor-bound protein 3; HT027; MST084; MSTP084; NCKAP2; Protein Ash; SH2/SH3 adapter GRB2 Specification REACTIVITY: H M R Hm Mk B SENSITIVITY: Endogenous MW (kDa): 25 SOURCE: Rabbit