CST, 3975S, G-alpha(o) Antibody

Polyclonal Antibody for studying Gnao. Validated for Western Blotting. Highly specific and rigorously validated in-house, G-alpha(o) Antibody (CST #3975) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity G-alpha(o) Antibody detects endogenous levels of total Gα(o) protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg15 of human Gα(o). Antibodies are purified by protein A and peptide affinity chromatography. Background Heterotrimeric guanine nucleotide-binding proteins (G proteins) consist of α, β and γ subunits and mediate the effects of hormones, neurotransmitters, chemokines, and sensory stimuli. To date, over 20 known Gα subunits have been classified into four families, Gα(s), Gα(i/o), Gα(q) and Gα(12), based on structural and functional similarities (1,2). Phosphorylation of Tyr356 of Gα(q)/Gα(11) is essential for activation of the G protein, since phenylalanine substitution for Tyr356 changes the interaction of Gα with receptors and abolishes ligand-induced IP formation (3). Gα(o) is the most abundant G protein in the brain and couples to serotonin, dopamine, GABA (B), opioid, glutamate and cholinergic receptors (4). Gα(o) -/- mice have neurological defects such as tremors, seizures and poor motor coordination (5). Alternate Names DKFZp686O0962; EIEE17; G protein subunit alpha o1; G-ALPHA-o; G-alpha(o); GNAO; GNAO1; GO2-q chimeric G-protein; guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O; guanine nucleotide binding protein, alpha activating polypeptide O; Guanine nucleotide-binding protein G(o) subunit alpha; guanine nucleotide-binding regulatory protein 2; HLA-DQB1; NEDIM Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 40 SOURCE: Rabbit