CST, 39774S, LOX (F2C8F) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying LOX. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, LOX (F2C8F) Rabbit Monoclonal Antibody (CST #39774) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity LOX (F2C8F) Rabbit Monoclonal Antibody recognizes endogenous levels of total LOX protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro282 of human LOX protein. Background Protein-lysin 6-oxidase (LOX) is a secreted copper-dependent amine oxidase and a member of the lysyl oxidase family (1). It primarily catalyzes the oxidation of lysine (or hydroxylysine) residues in collagen and elastin to form peptidyl aldehyde derivatives (2). These modifications are required for further cross-linking of target proteins to enhance extracellular matrix (ECM) stiffness. LOX plays critical roles in vascular, lung, and skin development, and tissue damage repair (3-5). Upregulation of LOX is associated with various diseases, including cancer progression and tissue fibrosis. Aberrant LOX activity creates a favorable tumor microenvironment to promote tumor metastasis and distal colonization (6-8). Alternate Names AAT10; LOX; LYOX; Lysyl oxidase; MGC105112; Protein-lysine 6-oxidase; Protein-lysine 6-oxidase, long form; Protein-lysine 6-oxidase, short form Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 56, 54 Source/Isotype: Rabbit IgG