CST, 3977S, CDX2 Antibody

Polyclonal Antibody for studying CDX2. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, CDX2 Antibody (CST #3977) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity CDX2 Antibody detects endogenous levels of total CDX2 protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids at the N-terminus of human CDX2. Antibodies are purified by Protein A and peptide affinity chromatography. Background CDX2, a homeobox domain-containing transcription factor, is a master regulator of the trophectoderm, the layer that gives rise to extra-embryonic tissues in mammalian development (1). CDX2 is also involved in intestinal development (2), and gain of expression or loss of expression has been associated with various human malignancies, such as Barrett's esophagus (3) and colorectal cancer (4,5). Mouse embryonic stem cells deficient in CDX2 display limited hematopoietic progenitor development and altered gene expression (6), pointing to a role for CDX2 in gene regulation. CDX2 is also implicated in the aberrant expression of genes in human AML cell lines (7). Alternate Names caudal type homeo box transcription factor 2; caudal type homeobox 2; caudal type homeobox transcription factor 2; Caudal-type homeobox protein 2; CDX-3; CDX2; CDX2/AS; CDX3; Homeobox protein CDX-2; homeobox protein miniCDX2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 38 SOURCE: Rabbit