CST, 39827S, BIN1 Antibody

Polyclonal Antibody for studying BIN1. Validated for Western Blotting. Highly specific and rigorously validated in-house, BIN1 Antibody (CST #39827) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody . Protocol Available protocols: Western Blotting Specificity / Sensitivity BIN1 Antibody recognizes endogenous levels of total BIN1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val405 of human BIN1 Isoform IIA protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Bridging integrator 1 (BIN1, AMPHL) is an adaptor protein and putative tumor suppressor expressed as multiple isoforms due to alternative splicing. The BIN1 protein was originally identified as a Myc box-interacting protein with structural similarity to the synaptic vesicle protein amphiphysin (1). BIN1 protein structure contains an amino-terminal amphipathic helix and a BAR domain that is involved in sensing membrane curvature. The protein also includes a Myc-binding domain and an SH3 domain, which are implicated in protein-protein interactions (1). Multiple BIN1 isoforms range in size from approximately 45 to 65 kDa, with the nuclear BIN1 isoform found mostly in skeletal muscle and the cytoplasmic IIA isoform expressed in axon initial segments and nodes of Ranvier of the brain (2,3). Corresponding gene mutations and incorrect splicing can lead to impaired BIN1 membrane-tabulating and protein binding activities, resulting in development of autosomal recessive centronuclear myopathy and myotonic dystrophy (4,5). Genome-wide association studies link the gene with late-onset Alzheimer disease (AD) and increased BIN1 mRNA expression is seen in AD brains (6,7). Alternate Names AMPH2; Amphiphysin II; Amphiphysin-like protein; AMPHL; BIN1; box dependant MYC interacting protein 1; Box-dependent myc-interacting protein 1; Bridging integrator 1; CNM2; DKFZp547F068; MGC10367; Myc box-dependent-interacting protein 1; SH3P9 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 78-80 SOURCE: Rabbit