CST, 3988S, GAD2 Antibody

Polyclonal Antibody for studying GAD2. Validated for Western Blotting. Highly specific and rigorously validated in-house, GAD2 Antibody (CST #3988) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity GAD2 Antibody detects endogenous levels of total GAD2 protein. Species Reactivity: Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide surrounding Ala150 of human GAD2. Antibodies are purified by peptide affinity chromatography. Background The enzyme glutamate decarboxylase (GAD) is responsible for the synthesis of the essential neurotransmitter gamma-aminobutyric acid (GABA) from L-glutamic acid (1). GAD1 (GAD67) and GAD2 (GAD65) are expressed in nervous and endocrine systems (2) and are thought to be involved in synaptic transmission (3) and insulin secretion (4), respectively. Autoantibodies against GAD2 may serve as markers for type I diabetes (5). Many individuals suffering from an adult onset disorder known as Stiff Person Syndrome (SPS) also express autoantibodies to GAD2 (6). Alternate Names 65 kDa glutamic acid decarboxylase; DCE2; GAD-65; GAD2; GAD65; Glutamate decarboxylase 2; glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa); Glutamate decarboxylase 65 kDa isoform; Glutamate decarboxylase-2 (pancreas); MGC161605; MGC161607 Specification REACTIVITY: M R SENSITIVITY: Endogenous MW (kDa): 60 SOURCE: Rabbit