CST, 39901S, IL-17A (D1X7L) Rabbit Monoclonal Antibody (PE Conjugate)

Monoclonal Antibody for studying IL17A mouse. Validated for Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, IL-17A (D1X7L) Rabbit Monoclonal Antibody (PE Conjugate) (CST #39901) is ready to ship. Product Usage Information Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity IL-17A (D1X7L) Rabbit Monoclonal Antibody (PE Conjugate) recognizes endogenous levels of total mouse IL-17A protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val49 of mouse IL-17A protein. Background The IL-17 family of cytokines consists of IL-17A-F, and their receptors include IL-17RA-RE (1). IL-17 cytokines are produced by a variety of cell types including the Th17 subset of CD4+ T cells, as well as subsets of γδ T cells, NK cells, and NKT cells (2). IL-17A and IL-17F, the most well-studied of the IL-17 cytokines, contribute to fungal and bacterial immunity by inducing expression of proinflammatory cytokines, chemokines, and antimicrobial peptides (2). In addition, IL-17A contributes to the pathogenesis of several autoimmune diseases (3). IL-17E promotes Th2 cell responses (4). The roles of IL-17B, IL-17C, and IL-17D are less clear, however these family members also appear to have the capacity to induce proinflammatory cytokines (1,5,6). IL-17 receptors have an extracellular domain, a transmembrane domain, and a SEFIR domain. They are believed to signal as homodimers, heterodimers, or multimers through their SEFIR domain by recruiting the SEFIR domain-containing adaptor Act1 (7). Unlike most cytokines that signal through Jak/STAT pathways, IL-17 signaling results in NF-κB activation (8). Alternate Names Ctl; Ctla; CTLA-8; Ctla8; Cytotoxic T-lymphocyte-associated antigen 8; IL-; IL-17; IL-17A; Il17; Il17a; interleukin 17A; Interleukin-17A Specification REACTIVITY: M SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG