CST, 39909T, MRP3/ABCC3 (D8V8J) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ABCC3. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, MRP3/ABCC3 (D8V8J) Rabbit Monoclonal Antibody (CST #39909) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity MRP3/ABCC3 (D8V8J) Rabbit Monoclonal Antibody recognizes endogenous levels of total MRP3 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein surrounding Gly870 of human MRP3 protein. Background MRP3/ABCC3 belongs to the super family of ATP-binding cassette (ABC) transporters. It is a member of the MRP subfamily that is expressed in various organs including liver, gallbladder, small intestine, colon, kidney, and adrenal gland (1-3). MRP3 is involved in multi-drug resistance (1). It facilitates the efflux of organic anions including monoanionic bile acid and anti-cancer reagents such as etoposide and paclitaxel from liver and small intestine into blood (4-7). Expression of MRP3 is increased in the cholestatic human and rat liver, suggesting its role in cholehepatic and enterohepatic bile circulation and in protecting liver from toxic bile salts (2,8). MRP3 expression is also upregulated in people with Dubin-Johnson Syndrome (DJS) who lack functional MRP2 in the liver, which implicates the compensatory role of MRP3 in the absence of functional MRP2 (4). Elevated expression of MRP3 has been detected in various cancer types such as hepatocellular carcinomas, primary ovarian cancer, and adult acute lymphoblastic leukemia (ALL) (9-11). Overexpression of MRP3 was reported to be a prognostic factor in ALL and adult acute myeloid leukemia (AML) (11,12). Alternate Names ABC31; ABCC3; ATP binding cassette subfamily C member 3; ATP-binding cassette sub-family C member 3; ATP-binding cassette, sub-family C (CFTR/MRP), member 3; Canalicular multispecific organic anion transporter 2; canicular multispecific organic anion transporter; CMOAT2; DKFZp686E22157; EST90757; MLP2; MOAT-D; MRP3; Multi-specific organic anion transporter D; multidrug resistance associated protein; Multidrug resistance-associated protein 3 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 160-220 Source/Isotype: Rabbit IgG