CST, 39913S, HMGCL (E5H7V) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying HMGCL. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, HMGCL (E5H7V) Rabbit Monoclonal Antibody (CST #39913) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity HMGCL (E5H7V) Rabbit Monoclonal Antibody recognizes endogenous levels of total HMGCL protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly100 of human HMGCL protein. Background Mitochondrial hydroxymethylglutaryl-CoA lyase (HMGCL) catalyzes the conversion of 3-hydroxy-3-methylglutaryl-CoA to acetyl-CoA and acetoacetate (a ketone body). It is a key enzyme in ketogenesis (1,2). Studies show that HMGCL expression increases in BRAF V600E-expressing human melanoma cells, resulting in upregulated levels of intracellular acetoacetate. Acetoacetate enhances binding of BRAF V600E to MEK1, stimulating activation of the MEK-ERK signaling pathway. Suppression of HMGCL leads to reduced proliferation of these human melanoma cells (2). In addition, HMGCL and β-hydroxybutyrate (a ketone body) are shown to be essential to promote pancreatic ductal adenocarcinoma (PDA) tumor progression (3). Alternate Names 3-hydroxy-3-methylglutarate-CoA lyase; 3-hydroxy-3-methylglutaryl CoA lyase; 3-hydroxy-3-methylglutaryl-CoA lyase; 3-hydroxymethyl-3-methylglutaryl-CoA lyase; 3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase; HL; HMG-CoA lyase; HMGCL; hydroxymethylglutaricaciduria; Hydroxymethylglutaryl-CoA lyase, mitochondrial; mitochondrial 3-hydroxy-3-methylglutaryl-CoA lyase Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 30 Source/Isotype: Rabbit IgG