CST, 39958S, PLVAP Antibody (Carboxy-terminal Antigen)

Polyclonal Antibody for studying PLVAP. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, PLVAP Antibody (Carboxy-terminal Antigen) (CST #39958) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity PLVAP Antibody (Carboxy-terminal Antigen) recognizes endogenous levels of total PLVAP protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human PLVAP protein. Antibodies are purified by peptide affinity chromatography. Background PLVAP (plasmalemma vesicle-associated protein) is an endothelial cell (EC)-specific type II membrane protein. It forms diaphragms that bridge the opening of caveolae, fenestrae, and trans-endothelial channels and plays a role in regulating vascular permeability. PLVAP expression is restricted to a subset of ECs in the normal microvasculature, but it is not expressed in the ECs of large vessels, with the exception of the endocardial lining of the heart chambers (1). While PLVAP is expressed in ECs in the brain and retina during embryogenesis and postnatal development, it is not expressed in mature endothelium that forms blood-brain barrier (BBB) and blood-retinal barrier (BRB). However, under pathological conditions associated with barrier disruption such as brain ischemia, cancer, and diabetic retinopathy, PLVAP expression is induced in endothelium of BBB and BRB (2). PLVAP was previously considered as an EC marker of the vascular system, its expression is now reported in lymphatic ECs (LECs) in lymph nodes and it may play a role in regulating the parenchymal entry of lymphocytes and soluble antigens (3,4). Alternate Names DIAR10; FELS; Fenestrated endothelial-linked structure protein; fenestrated-endothelial linked structure protein; gp68; plasmalemma vesicle associated protein; Plasmalemma vesicle protein 1; Plasmalemma vesicle-associated protein; PLVAP; PV-1; PV-1 protein; PV1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 70, 140 SOURCE: Rabbit