CST, 3995S, MSH6 (P150) Antibody

Polyclonal Antibody for studying MSH6. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, MSH6 (P150) Antibody (CST #3995) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity MSH6 (P150) Antibody detects endogenous levels of total MSH6 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human MSH6. Antibodies are purified using protein A and peptide affinity chromatography. Background The DNA mismatch repair system (MMR) repairs post-replication DNA, inhibits recombination between nonidentical DNA sequences, and induces both checkpoint and apoptotic responses following certain types of DNA damage (1). MSH2 (MutS homologue 2) forms the hMutS-α dimer with MSH6 and is an essential component of the mismatch repair process. hMutS-α is part of the BRCA1-associated surveillance complex (BASC), a complex that also contains BRCA1, MLH1, ATM, BLM, PMS2 proteins, and the Rad50-Mre11-NBS1 complex (2). Mutations in MSH6 and other MMR proteins have been found in a large proportion of hereditary nonpolyposis colorectal cancer (Lynch Syndrome), the most common form of inherited colorectal cancer in the Western world (3). Mutations in MSH6 have been shown to occur in glioblastoma in response to temozolomide therapy and to promote temozolomide resistance (4). Alternate Names DNA mismatch repair protein Msh6; G/T mismatch-binding protein; GTBP; GTMBP; hMSH6; HNPCC5; HSAP; MSH6; mutS homolog 6; mutS homolog 6 (E. coli); MutS protein homolog 6; MutS-alpha 160 kDa subunit; p160; sperm-associated protein Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 160 SOURCE: Rabbit