CST, 40019SF, GNLY (E2T3D) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying GNLY. Validated for WB,IHC,IF,F. Highly specific and rigorously validated in-house, GNLY (E2T3D) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #40019) is ready to ship. Product Usage Information This product is the carrier free version of product #68091. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 68091 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity GNLY (E2T3D) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total GNLY protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human GNLY protein. Background Granulysin (GNLY) was originally identified as a late activation marker in T cells, and it is expressed by killer lymphocytes in most mammals, but not rodents. GNLY is largely confined to cytotoxic granules but can be secreted by killer cells, especially those expressing high levels of GNLY, such as decidual natural killer cells (1-3). GNLY is produced as a 15 kDa protein and is processed into a 9 kDa active pore-forming fragment by proteolytic removal of peptides from both the N- and C-termini. Moreover, the 15 kDa form was reported to function as an immune alarmin, causing the maturation and migration of antigen-presenting cells and other cells of the immune system (4-7). Unlike perforin, a cholesterol-dependent pore-forming protein that preferentially permeabilizes mammalian membranes, GNLY is inhibited by cholesterol and forms pores much more efficiently in microbial than mammalian membranes, and it plays an important role against bacteria, fungi, and parasite infections (8,9). Alternate Names D2S69E; GNLY; Granulysin; LAG-2; LAG2; lymphocyte-activation gene 2; Lymphokine LAG-2; NKG5; Protein NKG5; T-cell activation protein 519; T-lymphocyte activation gene 519; TLA519 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 15 Source/Isotype: Rabbit IgG