CST, 4002S, Troponin I Antibody

Polyclonal Antibody for studying Troponin-1/TNNI3. Validated for Western Blotting. Highly specific and rigorously validated in-house, Troponin I Antibody (CST #4002) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Troponin I Antibody detects endogenous levels of total troponin I, both skeletal muscle and cardiac isoforms. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with two synthetic peptides corresponding to the sequence of human troponin I. Antibodies are purified by protein A and peptide affinity chromatography. Background Troponin, working in conjunction with tropomyosin, functions as a molecular switch that regulates muscle contraction in response to changes in the intracellular Ca concentration. Troponin consists of three subunits: the Ca -binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI) (1). In response to β-adrenergic stimulation of the heart, Ser23 and Ser24 of TnI (cardiac) are phosphorylated by PKA and PKC. This phosphorylation stimulates a conformational change of the regulatory domain of TnC, reduces the association between TnI and TnC, and decreases myofilament Ca sensitivity by reducing the Ca binding affinity of TnC (1-3). Alternate Names Cardiac troponin I; cardiomyopathy, dilated 2A (autosomal recessive); CMD1FF; CMD2A; CMH7; cTnI; familial hypertrophic cardiomyopathy 7; MGC116817; RCM1; TNNC1; TNNI3; troponin I type 3 (cardiac); Troponin I, cardiac muscle; troponin I3, cardiac type Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 28 SOURCE: Rabbit