CST, 40090S, Calnexin (C5C9) Rabbit Monoclonal Antibody (HRP Conjugate)

Monoclonal Antibody for studying Calnexin. Validated for Western Blotting. Highly specific and rigorously validated in-house, Calnexin (C5C9) Rabbit Monoclonal Antibody (HRP Conjugate) (CST #40090) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 136 mM NaCl, 2.6 mM KCl, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Calnexin (C5C9) Rabbit Monoclonal Antibody (HRP Conjugate) detects endogenous levels of total calnexin protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding residues surrounding Pro52 of human calnexin protein. Background Secretory and transmembrane proteins are synthesized on polysomes and translocate into the endoplasmic reticulum (ER) where they are often modified by the formation of disulfide bonds, amino-linked glycosylation and folding. To help proteins fold properly, the ER contains a pool of molecular chaperones including calnexin. Calnexin was first identified as being involved in the assembly of murine class I histocompatibility molecules (1,2). Calnexin is a calcium-binding protein embedded in the ER membrane that retains the newly synthesized glycoproteins inside the ER to ensure proper folding and quality control (3-5). The specificity of calnexin for a subset of glycoproteins is defined by a lectin site, which binds an early oligosaccharide intermediate on the folding glycoprotein (5). Alternate Names Calnexin; CALX; CANX; CNX; epididymis secretory sperm binding protein; FLJ26570; IP90; Major histocompatibility complex class I antigen-binding protein p88; p90 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 90 Source/Isotype: Rabbit IgG