CST, 40142T, TET1 (E5F1O) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TET1. Validated for Western Blotting,Chromatin IP,CUT&RUN. Available in 2 sizes. Highly specific and rigorously validated in-house, TET1 (E5F1O) Rabbit Monoclonal Antibody (CST #40142) is ready to ship. Product Usage Information For optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. The CUT&RUN dilution was determined using CUT&RUN Assay Kit # 86652 . Western Blotting: 1:1000 Chromatin IP: 1:50 CUT&RUN: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Chromatin IP, CUT&RUN Specificity / Sensitivity TET1 (E5F1O) Rabbit Monoclonal Antibody recognizes endogenous levels of total TET1 protein. This antibody may detect a nonspecific band at 60 kDa. Species Reactivity: Human, Mouse, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human TET1 protein. Background Methylation of DNA at cytosine residues is a heritable, epigenetic modification that is critical for proper regulation of gene expression, genomic imprinting, and mammalian development (1,2). 5-methylcytosine is a repressive epigenetic mark established by two enzymes, DNMT3a and DNMT3b, and is maintained by DNMT1 (3,4). 5-methylcytosine was originally thought to be passively depleted during DNA replication. However, subsequent studies have shown that Ten-Eleven Translocation (TET) proteins TET1, TET2, and TET3 can catalyze the oxidation of methylated cytosine to 5-hydroxymethylcytosine (5-hmC) (5). Additionally, TET proteins can further oxidize 5-hmC to form 5-formylcytosine (5-fC) and 5-carboxylcytosine (5-caC), both of which are excised by thymine-DNA glycosylase (TDG), effectively linking cytosine oxidation to the base excision repair pathway and supporting active cytosine demethylation (6,7). TET1 is highly expressed in embryonic stem cells and is essential for maintaining stem cell pluripotency through demethylation of the promoter (8). Aberrant TET1 expression has also been implicated in a variety of cancers, including hepatocellular carcinoma, T-cell acute lymphoblastic leukemia (T-ALL), and triple-negative breast cancer (TNBC), among others (9-11). Alternate Names bA119F7.1; CXXC finger 6; CXXC zinc finger 6; CXXC-type zinc finger protein 6; CXXC6; FLJ10839; FLJ41442; KIAA1676; LCX; Leukemia-associated protein with a CXXC domain; Methylcytosine dioxygenase TET1; Ten-eleven translocation 1 gene protein; ten-eleven translocation-1; tet methylcytosine dioxygenase 1; tet oncogene 1; TET1; TET1 splice variant VP_DE4; TET1 splice variant VP_DE456 Specification REACTIVITY: H M Mk SENSITIVITY: Endogenous MW (kDa): 300 Source/Isotype: Rabbit IgG