CST, 40180S, PJA2 Antibody

Polyclonal Antibody for studying PJA2. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, PJA2 Antibody (CST #40180) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity PJA2 Antibody recognizes endogenous levels of total PJA2 protein. Species Reactivity: Human, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly332 of human PJA2 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Praja-2 (PJA2/RNF131) is a RING-H2-type E3 ubiquitin ligase. PJA2 is highly expressed in neural tissue and research studies have shown that PJA2 plays a role modulating synaptic archictecture and long-term memory by promoting the ubiquitin-dependent proteolysis of NOGO-A and PKA regulatory subunits (1,2). Research studies have also shown that PJA2 sustains gliobastoma growth by driving the ubiquitin-dependent proteasomal degradation of MOB, which inactivates the LATS tumor suppressor kinase of the Hippo pathway (3). Alternate Names E3 ubiquitin-protein ligase Praja-2; KIAA0438; Neurodap1; PJA2; praja 2, RING-H2 motif containing; praja ring finger 2; praja ring finger 2, E3 ubiquitin protein ligase; praja ring finger ubiquitin ligase 2; Praja2; RING finger protein 131; RING-type E3 ubiquitin transferase Praja-2; RNF131 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 150 SOURCE: Rabbit