CST, 40199T, UBAP2L (E5X4E) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying NICE-4. Validated for WB,IP,IHC,IF,eCLIP. Available in 2 sizes. Highly specific and rigorously validated in-house, UBAP2L (E5X4E) Rabbit Monoclonal Antibody (CST #40199) is ready to ship. Product Usage Information For more information about the RBP-eCLIP service please visit Eclipsebio . Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:200 - 1:800 Immunofluorescence (Immunocytochemistry): 1:50 - 1:200 eCLIP: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity UBAP2L (E5X4E) Rabbit Monoclonal Antibody recognizes endogenous levels of total UBAP2L protein. This antibody may detect non-specific bands of unknown origin between 80-110 kDa. Species Reactivity: Human, Mouse, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human UBAP2L protein. Background Ubiquitin-associated protein 2-like (UBAP2L) is a ubiquitous and highly conserved protein containing an N-terminal ubiquitin-associated (UBA) domain involved in the ubiquitin-proteasome system (UPS) and aggregate formation induced by proteasome inhibitors (1). It can also interact with the Polycomb group protein BMI1 to form a Polycomb subcomplex and regulate hematopoietic stem cell activity (2). UBAP2L is essential for the formation of stress granules (SGs), small cytosolic membraneless organelles that form in eukaryotic cells after stress exposure (3). Some studies suggest that UBAP2L is methylated by the arginine methyltransferase PRMT1, and that this methylation inhibits UBAP2L interaction with SG elements and overall SG assembly (4,5). UBAP2L is also an oncogenic protein found to be amplified and/or overexpressed in a large subset of cancers, including breast cancer, hepatocellular carcinoma, lung adenocarcinoma, glioblastoma, prostate cancer, and colorectal cancer, where it is often associated with tumor growth and poor prognosis (6-12). Alternate Names FLJ42300; KIAA0144; NICE-4; NICE4; Protein NICE-4; UBAP2L; ubiquitin associated protein 2 like; ubiquitin associated protein 2-like; Ubiquitin-associated protein 2-like; UBP2L Specification REACTIVITY: H M Mk SENSITIVITY: Endogenous MW (kDa): 150, 160 Source/Isotype: Rabbit IgG