CST, 4022S, MMP-2 Antibody

Polyclonal Antibody for studying MMP2. Validated for Western Blotting. Highly specific and rigorously validated in-house, MMP-2 Antibody (CST #4022) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity MMP-2 Antibody detects full length (proenzyme, 72 kDa) and cleaved (active enzyme, 64 kDa) MMP-2. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys116 of human MMP-2. Antibodies are purified by protein A and peptide affinity chromatography. Background The matrix metalloproteinases (MMPs) are a family of proteases that target many extracellular proteins including other proteases, growth factors, cell surface receptors, and adhesion molecules (1). Among the family members, MMP-2, MMP-3, MMP-7, and MMP-9 have been characterized as important factors for normal tissue remodeling during embryonic development, wound healing, tumor invasion, angiogenesis, carcinogenesis, and apoptosis (2-4). Research studies have shown that MMP activity correlates with cancer development (2). One mechanism of MMP regulation is transcriptional (5). Once synthesized, MMP exists as a latent proenzyme. Maximum MMP activity requires proteolytic cleavage to generate active MMPs by releasing the inhibitory propeptide domain from the full-length protein (5). Alternate Names 72 kDa gelatinase; 72 kDa type IV collagenase; CLG4; CLG4A; collagenase type IV-A; Gelatinase A; matrix metallopeptidase 2; matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase); Matrix metalloproteinase-2; matrix metalloproteinase-II; MMP-2; MMP-II; MMP2; MONA; neutrophil gelatinase; PEX; TBE-1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 64, 72 SOURCE: Rabbit