CST, 40291S, E2F4 (E3G2G) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying E2F4. Validated for Western Blotting,Immunoprecipitation,Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, E2F4 (E3G2G) Rabbit Monoclonal Antibody (CST #40291) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Chromatin IP: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Chromatin IP Specificity / Sensitivity E2F4 (E3G2G) Rabbit Monoclonal Antibody recognizes endogenous levels of total E2F4 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu405 of human E2F4 protein. Background The E2F family consists of 8 transcription factors that regulate genes that control cell cycle progression by complexing with DP and Rb proteins (1-4). E2F transcriptional activation is generally opposed by associating with RB proteins, pRB, p107, and p130 (5-7). E2F-1, -2, and -3a function as activators that can help quiescent cells enter S phase, while E2F-3b, -4, and -5 repress cell growth through the recruitment of HDAC's and other corepressors to target genes (8-10). E2F-6 diverges considerably from other family members, and has repressive properties governed not through interaction with Rb proteins, but by recruiting the polycomb repressive complex (11,12). E2F-7, and -8 are unique in that they have two DNA-binding domains and do not heterodimerize with DP proteins. These E2F family members repress transcription and delay progression of the cell cycle through the regulation of E2F-1 (13-15) Alternate Names E2F transcription factor 4; E2F transcription factor 4, p107/p130-binding; E2F-4; E2F4; p107/p130-binding protein; Transcription factor E2F4 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 62 Source/Isotype: Rabbit IgG