CST, 4032S, CaMKIV Antibody

Polyclonal Antibody for studying CaMK4. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, CaMKIV Antibody (CST #4032) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity CaMKIV Antibody detects total levels of endogenous CaMKIV. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide around amino acid 282-300 of human CaMKIV. Antibodies are purified by protein A and peptide affinity chromatography. Background CaMKIV is an important member of calcium/calmodulin-activated kinases. CaMKIV signaling has been related to long-term neural potentiation and memory (1,2), as well as T-cell receptor signaling (3). CaMKIV has catalytic and regulatory domains. The Ca2+/calmodulin-dependent CaMKK phosphorylates CaMKIV, releasing the autoinhibitory effect and thus activating the kinase (4-6). The activated CaMKIV further autophosphorylates itself at Thr196 (or Thr200 in human) to render the kinase constitutively active (5). The threonine phosphorylation state of CaMKIV can be downregulated by PP2A dephosphorylation. Alternate Names brain Ca(2+)-calmodulin-dependent protein kinase type IV; brain Ca++-calmodulin-dependent protein kinase type IV; calcium/calmodulin dependent protein kinase IV; calcium/calmodulin-dependent protein kinase IV; Calcium/calmodulin-dependent protein kinase type IV; calcium/calmodulin-dependent protein kinase type IV catalytic chain; CAM kinase IV; CAM kinase- GR; CaM kinase-GR; CAMK; CaMK IV; CaMK-GR; CAMK4; CAMKIV; KCC4; MGC36771 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 58, 60: human. 61, 63: rat and mouse. SOURCE: Rabbit