CST, 40354S, Nectin-3/PVRL3 (F9R9R) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PVRL3. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Nectin-3/PVRL3 (F9R9R) Rabbit Monoclonal Antibody (CST #40354) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Nectin-3/PVRL3 (F9R9R) Rabbit Monoclonal Antibody recognizes endogenous levels of total nectin-3/PVRL3 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val521 of human nectin-3/PVRL3 protein. Background Nectin cell adhesion molecule 3 (Nectin-3), also known as poliovirus receptor-related protein 3 (PVRL3) and CD113, is a member of the immunoglobulin-like cellular adhesion molecule family and plays an important role in calcium-independent cellular adhesion (1,2). There are four nectin family members, nectin-1, nectin-2, nectin-3, and nectin-4. They are composed of an ectodomain containing three tandem Ig-like regions arranged as an N-terminal variable-like domain followed by two constant-like domains, a single transmembrane region, and a cytoplasmic domain that binds to the actin cytoskeleton through the adaptor protein afadin (3,4). Nectin-3 can engage in homophilic, as well as heterophilic cell adhesion by engaging with other nectin family members (5). In the nervous system, nectin-3 expression is associated with chronic stress-induced synaptic abnormalities and has been proposed as a target for stress-related cognitive disorder treatment (6,7). Nectin-3 can also interact with the immune system. Nectin-3 expressed on antigen-presenting cells (APCs) and tumor cells can act as a ligand for the inhibitory receptor TIGIT, expressed on activated T cell subsets and natural killer (NK) cells (8). Aberrant expression of nectin-3 has been observed in multiple cancers, including breast, pancreatic, lung, colorectal, and ovarian cancer (9-14). Alternate Names CD113; CDw113; DKFZp566B0846; FLJ90624; NECT3; nectin 3; Nectin cell adhesion molecule 3; Nectin-3; NECTIN3; poliovirus receptor-related 3; Poliovirus receptor-related protein 3; PPR3; PRR3; PVRL3; PVRR3 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 80-100 Source/Isotype: Rabbit IgG