CST, 4040S, Jak2 (D2E12) Rabbit Monoclonal Antibody (Biotinylated)

Monoclonal Antibody for studying Jak2. Validated for Western Blotting. Highly specific and rigorously validated in-house, Jak2 (D2E12) Rabbit Monoclonal Antibody (Biotinylated) (CST #4040) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 140 mM NaCl, 3 mM KCI, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Jak2 (D2E12) Rabbit Monoclonal Antibody (Biotinylated) detects endogenous levels of total Jak2 protein. No cross-reactivity was observed with other family members. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro841 of Jak2 protein. Background Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3, and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4), and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains. Alternate Names JAK-2; JAK2; Janus kinase 2; Janus kinase 2 (a protein tyrosine kinase); JTK10; Tyrosine-protein kinase JAK2 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 125 Source/Isotype: Rabbit IgG