CST, 4044T, HEF1/NEDD9 (2G9) Mouse Monoclonal Antibody

Monoclonal Antibody for studying NEDD9. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, HEF1/NEDD9 (2G9) Mouse Monoclonal Antibody (CST #4044) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 44305 . Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity HEF1/NEDD9 (2G9) Mouse Monoclonal Antibody detects endogenous levels of total HEF1/NEDD9 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant protein fragment corresponding to residues from the human HEF1/NEDD9 protein. Background Human enhancer of filamentation protein 1 (HEF1), also known as neural precursor cell expressed developmentally down-regulated protein 9 (NEDD9), is part of the Cas family of proteins, which include HEF1/NEDD9, p130Cas and Efs (1). HEF1 is a predominantly cytoplasmic protein, localizing to focal adhesions during interphase, and centrosomes and other parts of the mitotic apparatus during G2/M phase of the cell cycle (2). HEF1 is a docking protein that plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion, motility, growth and apoptosis (1). Phosphorylation of HEF1 is induced by a number of factors, including FAK, TGF-β, PDGFR, Abl, and BCR-ABL, which leads to coordinate binding of multiple downstream effector proteins via 15 known SH2 domain-binding sites (1). HEF1 is a key regulator of cancer metastasis. It is required for the invasive activity of glioblastomas (3), is linked to the promotion of metastasis in melanoma (4), and is found to be up-regulated in lung cancer metastasis (5). Alternate Names Cas scaffolding protein family member 2; CAS-L; cas-like docking; CAS2; CASL; CASS2; Crk-associated substrate related; Crk-associated substrate related protein Cas-L; CRK-associated substrate-related protein; dJ49G10.2; dJ49G10.2 (Enhancer of Filamentation 1 (HEF1)); dJ761I2.1; dJ761I2.1 (enhancer of filamentation (HEF1)); Enhancer of filamentation 1; Enhancer of filamentation 1 p55; hEF1; NEDD-9; NEDD9; Neural precursor cell expressed developmentally down-regulated protein 9; neural precursor cell expressed, developmentally down-regulated 9; p105; p130Cas-related protein; Renal carcinoma antigen NY-REN-12 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 105, 115 Source/Isotype: Mouse IgG1