CST, 40491S, M-Cadherin (D4B9L) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CDH15 mouse. Validated for WB,IP,IF,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, M-Cadherin (D4B9L) Rabbit Monoclonal Antibody (CST #40491) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Immunofluorescence (Frozen): 1:200 Immunofluorescence (Immunocytochemistry): 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 99890 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity M-Cadherin (D4B9L) Rabbit Monoclonal Antibody recognizes endogenous levels of total M-Cadherin protein. The lower band in the doublet shown in C2C12 and RD extracts is unglycosylated M-Cadherin. The identity of the ~78 kDa band that is present in the C2C12 extract is not known but is likely to be a breakdown product in the lysate as it is absent in the IP input and pull-down. Note that human M-Cadherin runs slightly lower than mouse M-Cadherin. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Alanine 399 of human M-Cadherin protein. Background Cadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellular domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development (1). The classic cadherin subfamily includes N-, P-, R-, B-, and E-cadherins, as well as about ten other members that are found in adherens junctions, a cellular structure near the apical surface of polarized epithelial cells. The cytoplasmic domain of classical cadherins interacts with β-catenin, γ-catenin (also called plakoglobin), and p120 catenin. β-catenin and γ-catenin associate with α-catenin, which links the cadherin-catenin complex to the actin cytoskeleton (1,2). While β- and γ-catenin play structural roles in the junctional complex, p120 regulates cadherin adhesive activity and trafficking (1-4). Investigators consider E-cadherin an active suppressor of invasion and growth of many epithelial cancers (1-3). Research studies indicate that cancer cells have upregulated N-cadherin in addition to loss of E-cadherin. This change in cadherin expression is called the "cadherin switch." N-cadherin cooperates with the FGF receptor, leading to overexpression of MMP-9 and cellular invasion (3). Research studies have shown that in endothelial cells, VE-cadherin signaling, expression, and localization correlate with vascular permeability and tumor angiogenesis (5,6). Investigators have also demonstrated that expression of P-cadherin, which is normally present in epithelial cells, is also altered in ovarian and other human cancers (7,8). M-cadherin is a cell-cell adhesion molecule expressed in satellite cells, a collection of adult stem cells/myogenic progenitors found in mature muscle tissue (9). Research studies indicate that M-cadherin may be involved in the recognition and fusion of adjacent cells, and may play an important role in activating satellite cell division (10). Alternate Names AI323380; CAD15; cadherin 15; Cadherin-14; Cadherin-15; Cdh14; Cdh15; M cadherin; M-cadherin; Mca; Mcad; Muscle cadherin Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 130 Source/Isotype: Rabbit IgG