CST, 4049S, THEX1 Antibody

Polyclonal Antibody for studying THEX1 mouse. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, THEX1 Antibody (CST #4049) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity THEX1 Antibody detects endogenous levels of total THEX1 protein. Species Reactivity: Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala337 of mouse THEX1. Antibodies are purified by protein A and peptide affinity chromatography. Background THEX1 (3'hExo) is a 3' exonuclease that may play a role in the degradation of histone mRNA transcripts (1). A recently identified member of the DEDDh 3' exonuclease family, THEX1 binds the conserved stem-loop structure found at the 3' end of mRNA (2). The binding of THEX1 to mRNA requires the presence of a terminal ACCCA sequence and is enhanced by the concurrent binding of stem-loop binding protein (SLBP). Cleavage of histone mRNA by THEX1 exonuclease may help produce the rapid turnover of histone mRNA transcripts associated with the completion of DNA replication (3). Additional evidence suggests that THEX1 may be responsible for excising the remaining few 3' nucleotides following cleavage by a different enzyme (4). Alternate Names 3'-5' exonuclease ERI1; 3'-5' exoribonuclease 1; 3'exo; 3'hexo; 3110010F15Rik; enhanced RNAi protein 1; eri-1; Eri-1 homolog; Eri1; exoribonuclease 1; histone mRNA 3' end-specific exonuclease; Histone mRNA 3'-exonuclease 1; Thex1; three prime histone mRNA exonuclease 1 Specification REACTIVITY: M R SENSITIVITY: Endogenous MW (kDa): 42 SOURCE: Rabbit