CST, 40538S, Glut3 (E7M7V) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Glut3. Validated for WB,IHC,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, Glut3 (E7M7V) Rabbit Monoclonal Antibody (CST #40538) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:3200 - 1:12800 Immunofluorescence (Immunocytochemistry): 1:800 - 1:1600 Flow Cytometry (Fixed/Permeabilized): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 18035 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Glut3 (E7M7V) Rabbit Monoclonal Antibody recognizes endogenous levels of total Glut3 protein. This antibody does not cross-react with Glut1, Glut2, or Glut4. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala472 of human Glut3 protein. Background Glucose transporter 3 (Glut3, also known as SLC2A3) has a higher affinity for glucose than the ubiquitous glucose transporter Glut1. In the normal brain, Glut3 expression is limited to neurons, whereas Glut1 is expressed in both neurons and glia. Glioblastoma-derived brain tumor initiating cells (BTICs) express increased levels of Glut3, enabling their survival in the microenvironment deficient in glucose. In addition, Glut3 expression levels correlate with poor survival in brain tumors (1). Furthermore, integrin αvβ3 was shown to stimulate PAK4-YAP/TAZ signaling to upregulate Glut3 expression in glioblastoma cells. A subpopulation of glioblastoma was identified to be addicted to Glut3 and is highly sensitive to the disruption of integrin αvβ3-PAK4-YAP/TAZ signaling (2,3). Alternate Names FLJ90380; Glucose transporter type 3, brain; GLUT-3; GLUT3; GTR3; SLC2A3; solute carrier family 2 (facilitated glucose transporter), member 3; solute carrier family 2 member 3; Solute carrier family 2, facilitated glucose transporter member 3 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 50-70 Source/Isotype: Rabbit IgG