CST, 4055S, THEX1 (D66A11) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying THEX1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, THEX1 (D66A11) Rabbit Monoclonal Antibody (CST #4055) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity THEX1 (D66A11) Rabbit Monoclonal Antibody detects endogenous levels of total THEX1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence around Pro33 of human THEX1. Background THEX1 (3'hExo) is a 3' exonuclease that may play a role in the degradation of histone mRNA transcripts (1). A recently identified member of the DEDDh 3' exonuclease family, THEX1 binds the conserved stem-loop structure found at the 3' end of mRNA (2). The binding of THEX1 to mRNA requires the presence of a terminal ACCCA sequence and is enhanced by the concurrent binding of stem-loop binding protein (SLBP). Cleavage of histone mRNA by THEX1 exonuclease may help produce the rapid turnover of histone mRNA transcripts associated with the completion of DNA replication (3). Additional evidence suggests that THEX1 may be responsible for excising the remaining few 3' nucleotides following cleavage by a different enzyme (4). Alternate Names 3' exoribonuclease; 3'-5' exonuclease ERI1; 3'-5' exoribonuclease 1; 3'EXO; 3'HEXO; enhanced RNAi three prime mRNA exonuclease homolog 1; Eri-1 homolog; ERI1; exoribonuclease 1; HEXO; histone mRNA 3' end-specific exonuclease; Histone mRNA 3'-end-specific exoribonuclease; Histone mRNA 3'-exonuclease 1; MGC35395; Protein 3'hExo; THEX1; three prime histone mRNA exonuclease 1; three prime mRNA exonuclease 1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 46 Source/Isotype: Rabbit IgG