CST, 40584S, CD57 (HNK-1) Mouse Monoclonal Antibody (Alexa Fluor® 647 Conjugate)

Monoclonal Antibody for studying . Validated for Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized),Flow Cytometry (Live). Highly specific and rigorously validated in-house, CD57 (HNK-1) Mouse Monoclonal Antibody (Alexa Fluor^® 647 Conjugate) (CST #40584) is ready to ship. Product Usage Information Immunofluorescence (Immunocytochemistry): 1:50 - 1:200 Flow Cytometry (Fixed/Permeabilized): 1:50 Flow Cytometry (Live): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. This product is stable for 12 months when stored at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized), Flow Cytometry (Live) Specificity / Sensitivity CD57 (HNK-1) Mouse Monoclonal Antibody (Alexa Fluor Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a membrane antigen from HSB-2 cells. Background CD57 antigen, also known as HNK-1 and Leu7, is a terminally sulfated glycan carbohydrate epitope (glyco-epitope) expressed on a variety of proteins, lipids, and chondroitin sulfate proteoglycans at the cell surface (1,2). CD57 is synthesized by the enzyme B3GAT1 and is present on a subset of peripheral blood lymphocytes, including NK cells and CD8 T cells, as well as neural cells and striated muscle (3-5). Studies have shown that CD57 is not expressed on platelets, monocytes, granulocytes, or red blood cells (6). The CD57 epitope may play a role in neural cell adhesion (7), and characterizes unique maturation states in T and NK cells (8,9). CD57 is an important marker when studying functional immune deficiency in patients with autoimmune diseases, infectious diseases, and cancers (1). Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Mouse IgM