CST, 40592S, Phospho-Atg4B (Ser383) (E3I8M) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ATG4B (Ser383) phosphate. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Atg4B (Ser383) (E3I8M) Rabbit Monoclonal Antibody (CST #40592) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Phospho-Atg4B (Ser383) (E3I8M) Rabbit Monoclonal Antibody recognizes endogenous levels of Atg4B protein only when phosphorylated at Ser383. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser383 of human Atg4B protein. Background Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Control of autophagy was largely discovered in yeast and involves proteins encoded by a set of autophagy-related genes (Atg) (1). Formation of autophagic vesicles requires a pair of essential ubiquitin-like conjugation systems, Atg12-Atg5 and Atg8-phosphatidylethanolamine (Atg8-PE), which are widely conserved in eukaryotes (2). Numerous mammalian counterparts to yeast Atg proteins have been described, including three Atg8 proteins (GATE-16, GABARAP, and LC3) and four Atg4 homologs (Atg4A/autophagin-2, Atg4B/autophagin-1, Atg4C/autophagin-3, and Atg4D/autophagin-4) (3-5). The cysteine protease Atg4 is pivotal to autophagosome membrane generation and regulation. Atg4 primes the Atg8 homolog for lipidation by cleaving its carboxy terminus and exposing its glycine residue for E1-like enzyme Atg7. The Atg8 homolog is transferred to the E2-like enzyme Atg3 before forming the Atg8-PE conjugate. During later stages of autophagy, Atg4 can reverse this lipidation event by cleaving PE, thereby recycling the Atg8 homolog (6). Phosphorylation of Atg4B by MST4 increases its activity and autophagic flux (7). Alternate Names APG4 autophagy 4 homolog B; APG4B; ATG4 autophagy related 4 homolog B; ATG4 autophagy related 4 homolog B (S. cerevisiae); ATG4B; AUT-like 1 cysteine endopeptidase; AUTL1; Autophagin-1; autophagy related 4B cysteine peptidase; Autophagy-related cysteine endopeptidase 1; Autophagy-related protein 4 homolog B; Cysteine protease ATG4B; hAPG4B; KIAA0943; MGC1353 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 48 Source/Isotype: Rabbit IgG