CST, 40623T, CD6 (E9Y7Y) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CD6. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, CD6 (E9Y7Y) Rabbit Monoclonal Antibody (CST #40623) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 36549 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity CD6 (E9Y7Y) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD6 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human CD6 protein. Background T cell differentiation antigen CD6 is a cell adhesion molecule expressed on immature thymocytes and mature T cells, and has also been detected on a subset of B cells and NK cells within the immune system (1-4). CD6 mediates cell-cell interactions through its binding partner CD166/ALCAM (2), and contributes to the formation and maturation of the immunological synapse (3,4). CD6 functions as a co-stimulatory receptor, promoting T cell activation and proliferation through the TCR/CD3 complex signaling cascade (3-6). Studies have shown CD6 can be glycosylated (7), hyperphosphorylated on serine and threonine residues (8), and phosphorylated on tyrosine residues (6,9), each of which can differentially affect the function and signaling of this molecule. CD6 also functions as a calcium-dependent pattern receptor that binds and aggregates Gram-positive and Gram-negative bacteria. In response to lipopolysaccharide, CD6 mediates activation of the inflammatory response and secretion of pro-inflammatory cytokines (10). Alternate Names CD6; CD6 antigen; CD6 molecule; FLJ44171; Soluble CD6; T-cell differentiation antigen CD6; T12; TP120 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 100-140 Source/Isotype: Rabbit IgG