CST, 40653T, PHF6 (E8F2L) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PHF6. Validated for Western Blotting,Immunoprecipitation,Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, PHF6 (E8F2L) Rabbit Monoclonal Antibody (CST #40653) is ready to ship. Product Usage Information For optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Immunoprecipitation: 1:100 Chromatin IP: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Chromatin IP Specificity / Sensitivity PHF6 (E8F2L) Rabbit Monoclonal Antibody recognizes endogenous levels of total PHF6 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu269 of human PHF6 protein. Background PHD finger protein 6 (PHF6) is a 41 kDa transcriptional repressor that was first identified as a mutated gene in Börjeson-Forssman-Lehmann syndrome (BFLS), an X-linked intellectual disability disorder (1,2). Somatic loss-of-function mutations in the gene have also been linked to T-cell acute lymphoblastic leukemia (T-ALL) and acute myeloid leukemia (AML) (3-5). Structurally, PHF6 contains two nuclear localization sequences, one nucleolar localization sequence, and two plant homeodomain (PHD)-like zinc fingers (6,7). Unlike other PHD proteins, the PHD domains of PHF6 are considered to be imperfect and have not been shown to directly bind to histones; however, the isolated second PHD domain (PHD2) has been shown to bind dsDNA directly (7). A more recent study finds that PHF6 interacts with histones via protein-protein interactions, and that this association is independent of DNA and enriched in the presence of the activating marks H3K27ac and H3K4me3 (8). PHF6 interacts with PAF1 and other subunits of the PAF1 transcription elongation complex, and knockdown of either PHF6 or PAF1 adversely affects proper neuronal positioning and migration in mouse cerebral cortex (9). PHF6 has also been shown to associate with members of the nucleosome remodeling and histone deacetylase (NuRD) chromatin remodeling complex, including CHD4, HDAC1, and RBBP4, where it is likely involved in transcriptional repression of developmental genes (10). PHF6 plays a critical role in regulating hematopoiesis, particularly by regulating chromatin accessibility to lineage-specific transcription factors. Studies suggest that PHF6 promotes B-cell lineage differentiation through the expression of B-cell specific genes, while simultaneously suppressing T-cell lineage differentiation (8). Indeed, a CRISPR-Cas9 knockout study shows that PHF6 is required for growth of B-ALL cells, while mice transplanted with PHF6-deficient B-ALL cells develop T-ALL phenotypes (8). Alternate Names BFLS; BORJ; CENP-31; centromere protein 31; KIAA1823; MGC14797; PHD finger protein 6; PHD-like zinc finger protein; PHF6; truncated PHF6 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 45 (human/monkey), 41 (mouse/rat) Source/Isotype: Rabbit IgG