CST, 40674S, KLF5 (E5K6H) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying KLF5. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, KLF5 (E5K6H) Rabbit Monoclonal Antibody (CST #40674) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:800 - 1:3200 Flow Cytometry (Fixed/Permeabilized): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity KLF5 (E5K6H) Rabbit Monoclonal Antibody recognizes endogenous levels of total KLF5 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu140 of human KLF5 protein. Background Kruppel-like factors (KLFs) are a family of zinc finger DNA-binding proteins that share homology to Kruppel transcription factor in (1). These transcription regulators play diverse roles in cell proliferation, differentiation, inflammation, and pluripotency (2). Various KLFs, including KLF5, are involved in cancer-related processes, such as epithelial-mesenchymal transition (EMT), invasion, and metastasis (3-6). In addition, studies show that KLF5 deficiency specific to renal collecting duct epithelial cells impairs cardiac stress adaptation response coordinated by the heart and kidney (7). Alternate Names (intestinal Kruppel-like factor; basic transcription element binding protein 2; Basic transcription element-binding protein 2; BTE-binding protein 2; BTEB2; CKLF; Colon krueppel-like factor; colon kruppel-like factor; epididymis secretory sperm binding protein; GC box binding protein 2; GC-box-binding protein 2; IKLF; Intestinal-enriched krueppel-like factor; intestinal-enriched kruppel-like factor; KLF5; Krueppel-like factor 5; Kruppel like factor 5; Kruppel-like factor 5 (intestinal); Transcription factor BTEB2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 60 Source/Isotype: Rabbit IgG