CST, 40737T, MEK5 (E4C4B) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MEK5. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, MEK5 (E4C4B) Rabbit Monoclonal Antibody (CST #40737) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity MEK5 (E4C4B) Rabbit Monoclonal Antibody recognizes endogenous levels of total MEK5 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly127 of human MEK5 protein. Background MEK5 is a dual specific kinase that specifically activates ERK5 involved in the regulation of diverse cellular processes, including cell growth, survival, and differentiation (1). The MEK5-ERK5 pathway is activated in numerous cancer types and plays a role in tumor growth and metastasis (2,3). Pharmacological targeting of the MEK5-ERK5 pathway has been investigated as a strategy for cancer treatment (4). MEK5 is activated by a variety of stimuli, including growth factors, cytokines, and oxidative stress (5,6). Activation of MEK5 is triggered by upstream kinases MEKK2 and MEKK3, leading to phosphorylation of MEK5 at Ser311 and Thr315, which subsequently leads to MEK5 dependent phosphorylation of EKR5 at Thr218 and Tyr220 (7,8). Phosphorylation of ERK5 leads to nuclear translocation and regulation of several oncogenic transcription factors, including MEF2C, c-Fos, c-Myc, and Sap1 (9-12). Alternate Names Dual specificity mitogen-activated protein kinase kinase 5; HsT17454; MAP kinase kinase 5; MAP kinase kinase MEK5b; MAP2K5; MAPK/ERK kinase 5; MAPKK 5; MAPKK5; MEK 5; MEK5; mitogen-activated protein kinase kinase 5; MKK5; MP2K5; PRKMK5 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 48 Source/Isotype: Rabbit IgG