CST, 40765S, eIF3D (E4I4S) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying eIF3D. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, eIF3D (E4I4S) Rabbit Monoclonal Antibody (CST #40765) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity eIF3D (E4I4S) Rabbit Monoclonal Antibody recognizes endogenous levels of total eIF3D protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro298 of human eIF3D protein. Background Translation initiation requires a set of factors to facilitate the association of the 40S ribosomal subunit with mRNA. The eIF4F complex, consisting of eIF4E, eIF4A, and eIF4G, binds to the 5' cap structure of mRNA. eIF4F and eIF4B unwind the secondary structure of mRNA at its 5' untranslated region. The 40S ribosomal subunit, along with some initiation factors including eIF3, then binds to the 5' mRNA cap and searches along the mRNA for the initiation codon. eIF3 is a large translation initiation complex with 10 to 13 different subunits. eIF3A, eIF3B, eIF3C, eIF3E, eIF3F, and eIF3H are the core subunits critical for the function of this complex. eIF3 physically interacts with eIF4G, which may be responsible for the association of the 40S ribosomal subunit with mRNA (1). eIF3D is another subunit of the eIF3 protein complex. It binds to the 5' cap structure of a subset of mRNAs. This eIF3D-mRNA interaction is required for the translation initiation complex formation on eIF3-specialized mRNAs (2). Studies found that eIF3D is activated under metabolic stress by decreased phosphorylation near its cap-binding pocket. The translation adaptation mediated by eIF3D is necessary for cell survival during glucose deprivation (3). Alternate Names eIF-3-zeta; eIF3 p66; eIF3-p66; eIF3-zeta; EIF3D; EIF3S7; Eukaryotic translation initiation factor 3 subunit 7; Eukaryotic translation initiation factor 3 subunit D; eukaryotic translation initiation factor 3, subunit 7 zeta, 66/67kDa; eukaryotic translation initiation factor 3, subunit D; MGC126526; MGC17258; translation initiation factor eIF3 p66 subunit Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 64 Source/Isotype: Rabbit IgG