CST, 40826SF, FoxM1 (D3F2B) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying FOXM1. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, FoxM1 (D3F2B) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #40826) is ready to ship. Product Usage Information This product is the carrier free version of product #20459. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 20459 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity FoxM1 (D3F2B) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total FoxM1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val699 of human FoxM1 protein. Background Forkhead box M1 (FoxM1) is a forkhead box family transcription factor that regulates a number of genes throughout the cell cycle to help control DNA replication, mitosis, and cell proliferation. FoxM1 expression increases during G1 and S and reaches maximum levels in G2/M (1-3). Nuclear translocation occurs just before entry into G2/M and is associated with FoxM1 phosphorylation (4). Phosphorylation of FoxM1 by MAPK (Ser331, Ser704), Cyclin/Cdk (Ser4, Ser35, Thr600, Thr611, Thr620, Thr627, Ser638), Plk1 (Ser715, Ser724), and Chk2 (Ser376) stabilizes and activates FoxM1 (4-8). Forkhead box M1 is expressed in all embryonic tissues but is restricted to proliferating tissues in adults (9). Research studies show that FoxM1 expression is negatively regulated by p53 (10,11). Upregulation of FoxM1 is associated with many human cancers, including prostate, breast, lung, ovary, colon, pancreas, stomach, bladder, liver, and kidney, and may be associated with p53 mutations in some tumors (11,12). As a result, FoxM1 inhibitors have become a topic of interest for potential cancer therapy (13). Alternate Names FKHL16; forkhead box M1; forkhead box M1-D; Forkhead box protein M1; forkhead-like 16; Forkhead-related protein FKHL16; Forkhead, drosophila, homolog-like 16; FOXM1; FOXM1A; FOXM1B; FOXM1C; Hepatocyte nuclear factor 3 forkhead homolog 11; HFH-11; HFH11; HNF-3; HNF-3/fork-head homolog 11; INS-1; M-phase phosphoprotein 2; MPHOSPH2; MPM-2 reactive phosphoprotein 2; MPP-2; MPP2; PIG29; TGT3; Transcription factor Trident; TRIDENT; WIN; Winged-helix factor from INS-1 cells Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 110 Source/Isotype: Rabbit IgG