CST, 40868T, CD40 (D8W3N) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CD40. Validated for WB,WB,IHC,IHC. Available in 2 sizes. Highly specific and rigorously validated in-house, CD40 (D8W3N) Rabbit Monoclonal Antibody (CST #40868) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 IHC Leica Bond: 1:200 Immunohistochemistry (Paraffin): 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 77841 . Protocol Available protocols: Western Blotting, IHC Leica Bond, Immunohistochemistry (Paraffin) Specificity / Sensitivity CD40 (D8W3N) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD40 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala135 of human CD40 protein. Background CD40, also known as tumor necrosis factor receptor superfamily member 5 (TNFRSF5), is a type I transmembrane protein expressed on the surface of B cells and professional antigen-presenting cells of the immune system, as well as on several non-hematopoietic cell types and cancers (1-4). CD40 interacts with CD40 ligand (CD40L/TNFSF5), which is expressed primarily on activated T cells but has also been reported on blood platelets, mast cells, basophils, NK cells, and B cells (5). Upon engagement with CD40L, CD40 signals through TNF receptor associated factors and MAP kinase signaling pathways, resulting in a wide variety of immune and inflammatory responses, including dendritic cell activation and cross-presentation, T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation (6-8). The CD40/CD40L axis is essential for the initiation and progression of cellular and humoral adaptive immunity, and is an important area of interest in the study of tumor immunology, neurodegenerative diseases, vascular diseases, and inflammatory disorders (9-12). Alternate Names B cell surface antigen CD40; B cell-associated molecule; B-cell surface antigen CD40; Bp50; CD40; CD40 antigen (TNF receptor superfamily member 5); CD40 molecule; CD40 molecule, TNF receptor superfamily member 5; CD40 type II isoform; CD40L receptor; CDw40; MGC9013; nerve growth factor receptor-related B-lymphocyte activation molecule; p50; TNFRSF5; TNR5; Tumor necrosis factor receptor superfamily member 5; tumor necrosis factor receptor superfamily, member 5 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 42 Source/Isotype: Rabbit IgG