CST, 4087S, Propidium Iodide (PI)/RNase Staining Solution

Cellular Dyes for studying in the research area. Product Usage Information As a last step for fluorescent assays, add enough neat (undiluted) Propidium Iodide (PI)/RNase Staining Solution to submerge cells or samples. Incubate for 15 minutes at room temperature, protected from light before analysis. Formulation Supplied in aqueous buffered solution containing less than 0.09% sodium azide. As with all intercalating DNA dyes, take all necessary safety precautions when using. Storage Store at 4°C protected from light, do not aliquot or dilute. The solution is stable for 12 months. Concentration: PI = 10ug/ml RNase = 300 ug/ml Background Propidium Iodide (PI) is a fluorescent dye which intercalates between bases and stains both DNA and RNA. Specific DNA staining is achieved by enzymatic removal of RNA with a ribonuclease (RNase). PI/RNase is commonly used as a nuclear stain in fluorescent microscopy and as a DNA content determinant in cell cycle analyses by flow cytometry. Cells in G2 and M phases of the cell cycle contain twice the DNA content compared to those in G0 and G1 phases. DNA content during S phase lies between these extremes. PI/RNase Staining Solution has excitation and emission maxima of 535 and 617 nm, respectively (orange to red range of the spectrum). This staining solution can be used in tandem with antibodies without binding interference and is a suitable DNA stain for multiplex assays. (1-3) Alternate Names cellular dyes; DNA dye